PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

ABSTRACT.- Romano L.A., Sampaio L.A. & Tesser M.B. 2012. [Micobacteriosis by Mycobacterium marinum in Brazilian flounder Paralichthys orbignyanus and barber goby Elacatinus figaro: Histopathological and imunohistochemical diagnosis.] Micobacteriose por Mycobacterium marinum em linguado Paralichthys orbignyanus e em “barber goby” Elacatinus figaro: diagnóstico histopatológico e imuno-histoquímico. Pesquisa Veterinária Brasileira 32(3):254-258. Laboratório de Imunologia Patologia de Organismos Aquáticos, Instituto de Oceanografia, Universidade Federal do Rio Grande, Av. Itália Km 8, Bairro Carreiros, Rio Grande, RS 96201-900, Brazil. E-mail: dcluis@yahoo.com Mycobacteriosis affects various species of fish, including those cultured with commercial purposes. It is a chronic systemic with granuloma forming disease known as fish mycobacteriosis. In the last few years the interest of fish culture has been increasing due to the reduction of the natural fish stocks. However the intensification of fish culture has collaborated to the development of diseases such as mycobacteriosis, causing losses due to the chronic characteristics and difficult diagnosis. This study is to report two cases of mycobacteriosis observed at “Laboratório de Piscicultura Estuarina e Marinha of the Universidade Federal do Rio Grande (FURG)”, one for Brazilian flounder Paralichthys orbignyanus and another for barber goby Elacatinus figaro. The Fite-Faraco staining protocol was used. Membranes of mycobacterial cells contain a waxy substance composed of mycolic acids. These are ß-hydroxy carboxylic acids with chain lengths of up to 90 carbon atoms. The property of acid fastness is related to the carbon chain length of the mycolic acid found in any particular species. The fish mycobacterium is much less acid and alcohol fast than the tubercle bacillus and the classic Ziehl-Neelsen Staining Protocol may give false results. The presence of mycobacterial antigen lesions was studied by immunohistological methods using monoclonal antibodies to Mycobacterium marinum. Humans may be infected by these bacteria and develop cutaneous granuloma. In this paper the necessity is stressed for a correct diagnosis of the disease, contributing to the prevention of its establishment in aquatic facilities, and thus, preserving cultured fish as well as human beings from infection.

• Micobacteriosis Mycobacterium marinum Paralichthys orbignyanus Elacatinus figaro micobacteriose

Abstract in Portuguese:

RESUMO.- Romano L.A., Sampaio L.A. & Tesser M.B. 2012. [Micobacteriosis by Mycobacterium marinum in Brazilian flounder Paralichthys orbignyanus and barber goby Elacatinus figaro: Histopathological and imunohistochemical diagnosis.] Micobacteriose por Mycobacterium marinum em linguado Paralichthys orbignyanus e em “barber goby” Elacatinus figaro: diagnóstico histopatológico e imuno-histoquímico. Pesquisa Veterinária Brasileira 32(3):254-258. Laboratório de Imunologia Patologia de Organismos Aquáticos, Instituto de Oceanografia, Universidade Federal do Rio Grande, Av. Itália Km 8, Bairro Carreiros, Rio Grande, RS 96201-900, Brazil. E-mail: dcluis@yahoo.com As micobacterioses afetam várias espécies de peixes, tanto comerciais como ornamentais. É uma enfermidade sistêmica com formação de granulomas, conhecida como micobacteriose de peixes. O desenvolvimento da aquicultura tem aumentado o número de casos com essa enfermidade que muitas vezes é de difícil diagnóstico. Neste trabalho apresentamos dois casos de micobacterioses em Paralichthys orbignyanus e Elacatinus figaro provenientes do Laboratório de Piscicultura Estuarina e Marinha da Universidade Federal do Rio Grande (FURG). As micobactérias de peixes possuem menos ácidos graxos que as de mamíferos, por isso foi utilizado o protocolo de coloração de Fite-Faraco, uma vez que o protocolo de coloração clássico de Ziehl Neelsen pode resultar em falsos negativos. Para determinar o tipo de micobactéria foi utilizada a imuno-histoquímica com a qual se pode diagnosticar a presença de Mycobacterium marinum. A enfermidade pode ser transmitida para o homem e com esse trabalho ressalta-se a necessidade de fazer o diagnóstico correto e implantar medidas de proteção para as pessoas que estão em contato com os peixes infectados.

Abstract in English:

ABSTRACT.- Romano L., Ferrígno C.R.A., Ferraz,V.C.M. Della Nina M., Ito K.C. 2008. [Evaluation of the use of interlocking nail and transcortical blockade for the repair of diaphyseal fractures of the femur in cats.] Avaliação do uso de haste bloqueada e bloqueio transcortical no reparo de fraturas diafisárias de fêmur em felinos. Pesquisa Veterinária Brasileira 28(4):201-206. Departamento de Cirurgia do Hospital Veterinário, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508-270, Brazil. E-mail: lromano@usp.br Interlocking nails allow rigid fracture stabilization and present biomechanical advantages when compared to other immobilization techniques. It acts along the central mechanical axis of the bone and preserves biological concept standards for osteosynthesis. The use of interlocking nail in cats is limited because the intramedullary space is relatively small for the available nail sizes. We reported 10 cases of cats that had simple or multiple closed fractures of the femoral diaphysis. The animals weighed 3.5 to 5 kg , which allowed using nails of 4.0mm and 4.7mm in diameter. For the nail insertion, retrograde and normograde routs were used, and four 2mm titanium screws were used to lock all the perforations. No trans-surgical complication occurred, however, due to a new trauma, one of the animals suffered femoral neck fracture and transversal distal fracture to the nail, three days after surgery. Radiographies were made up to four months after surgery, and in most cases secondary bone formation was seen, showing bone consolidation in 61-89 days, excluding the animal who suffered new trauma, who presented bone healing in 150 days. Clinically, occasional lameness occurred; however, except for the animal that had new trauma, all animals initiated adequate limb support in 3 to 8 days after surgery. In conclusion, the use of 4.0mm and 4.7mm interlocking nails in felines was adequate, with bone healing in all cases, and also return of good limb function after surgery.

• Fractures interlocking nail surgery cats

Abstract in Portuguese:

ABSTRACT.- Romano L., Ferrígno C.R.A., Ferraz,V.C.M. Della Nina M., Ito K.C. 2008. [Evaluation of the use of interlocking nail and transcortical blockade for the repair of diaphyseal fractures of the femur in cats.] Avaliação do uso de haste bloqueada e bloqueio transcortical no reparo de fraturas diafisárias de fêmur em felinos. Pesquisa Veterinária Brasileira 28(4):201-206. Departamento de Cirurgia do Hospital Veterinário, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508-270, Brazil. E-mail: lromano@usp.br Interlocking nails allow rigid fracture stabilization and present biomechanical advantages when compared to other immobilization techniques. It acts along the central mechanical axis of the bone and preserves biological concept standards for osteosynthesis. The use of interlocking nail in cats is limited because the intramedullary space is relatively small for the available nail sizes. We reported 10 cases of cats that had simple or multiple closed fractures of the femoral diaphysis. The animals weighed 3.5 to 5 kg , which allowed using nails of 4.0mm and 4.7mm in diameter. For the nail insertion, retrograde and normograde routs were used, and four 2mm titanium screws were used to lock all the perforations. No trans-surgical complication occurred, however, due to a new trauma, one of the animals suffered femoral neck fracture and transversal distal fracture to the nail, three days after surgery. Radiographies were made up to four months after surgery, and in most cases secondary bone formation was seen, showing bone consolidation in 61-89 days, excluding the animal who suffered new trauma, who presented bone healing in 150 days. Clinically, occasional lameness occurred; however, except for the animal that had new trauma, all animals initiated adequate limb support in 3 to 8 days after surgery. In conclusion, the use of 4.0mm and 4.7mm interlocking nails in felines was adequate, with bone healing in all cases, and also return of good limb function after surgery.